Experts spent four months making the life-size impression of the 26-year-old US singer.
He stands near a waxwork of his ex, Britney Spears, but as yet there are no plans to create a model of his other former love Cameron Diaz.
Madame Tussauds commissioned a Savile Row tailor to create a bespoke white suit for the star's waxwork at the London attraction.
Timberlake also features "old-school trainers" and designer stubble.
Timberlake, who began his UK tour last week, already has a waxwork from his 'N Sync boyband days in Madame Tussauds in the US.
A Madame Tussauds spokesman said: "Justin has been involved in the waxwork, selecting the images and the look that he wanted.
"He's one of those personalities that the public have been saying they would love to get up close and personal with for a long time."